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In 2D images, the actual length of the structure of interest (for example, a dendrite) can be difficult to trace because the structures can be mostly in shadow (dark) with little or no contrast. Also, it may not be practical to manually trace every structure in a 2D image. The most efficient approach is to automatically trace elongated structures based on a set of rules.
When a new 2D image is opened with NeuronJ, a dialog box appears (Figure 1A). First, it is necessary to place a cell of interest (for example, an individual neuron) in the selected area. After the cell is visible, the process is initiated.
Figure 1A: The dialog box for NeuronJ.
The user can specify the length of the segment to trace by clicking on the rules bar and entering the desired length. If a segment has already been traced, then the length can be changed using the ruler at the bottom.
This procedure is repeated until the desired amount of length has been traced.
Figure 1B: A neuron after the neuron is selected and the segment is traced (click on the green arrow).
NeuronJ performs various image processing functions as it traces the cell. For example, the background can be smoothed and the contrast enhanced, and cells of interest can be automatically thresholded. A sketch can be automatically generated to outline the traced cell. A special case is the generation of a contour map (Figure 1C) to visualize the elongated structures.
Figure 1C: A contour map can be generated automatically to display the elongated structures of the traced cell (click on the blue arrow).
NeuronJ Features:
• Automated Detection of Elongated Structures:
Automatic tracing is useful in 2D images because it eliminates the need for tracing and outlines of individual cells. It is also particularly useful for fluorescent images because the emission spectra of fluorescent markers differ significantly from the emission spectra of light in the visible spectrum. In such images, fluorescence with the same emission spectra often has a very different appearance from the background. However, when the cell is in the shadow, no structure can be traced.
To overcome this problem, NeuronJ automatically detects a cell of interest in the 2D image and traces the cell based on a series of rules. The rules can be configured for NeuronJ by placing all the rules on a rules bar.
NeuronJ takes into account the shape of the cell of interest 0cd6e936a3

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With this program you can create CSS menu, vertical and horizontal. You can specify color, font and setting for each menu item.
AJAX menu with ajax menu:
AJAX menu will allow you to create a menu with ajax or XML request.

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